Wolverine Research Stack 1.0
Products will arrive in a lyophilized (powder) form for maximum stability
$185.00
Wolverine Research Stack – Advanced Peptide Combination for Comprehensive Tissue Repair & Regeneration Studies
Includes:
The Wolverine Research Stack is a synergistic peptide combination specifically assembled for investigating advanced tissue regeneration, accelerated healing, and comprehensive cellular repair mechanisms. This powerful research stack leverages the complementary mechanisms of TB-500 and BPC-157 to create a multifaceted approach to studying:
Wound healing
Tissue remodeling
Inflammatory modulation
Musculoskeletal, gastrointestinal, and neurological repair
At 4Balance Peptides, we provide pharmaceutical-grade TB-500 and BPC-157 (≥98% purity each) in precisely measured, lyophilized 10mg vials, rigorously tested via HPLC and mass spectrometry to ensure optimal stability and research reliability. This combination product is intended exclusively for in vitro and animal model research under controlled laboratory conditions and is not for human or veterinary use.
Mechanism of Action – Synergistic Pathways
The TB-500 + BPC-157 stack exerts its effects through complementary and synergistic mechanisms:
Angiogenesis Enhancement
TB-500 promotes endothelial cell migration and vascular growth (Smart et al., 2007).
BPC-157 stimulates VEGF expression and new blood vessel formation (Seiwerth et al., 2018).
→ Together, they support superior vascularization compared to either compound alone.
Anti-Inflammatory Coordination
BPC-157 modulates NF-κB signaling and inflammatory cytokine expression (Sikiric et al., 2016).
TB-500 regulates actin polymerization and inflammatory cell migration (Philp et al., 2004).
→ This combination provides broad inflammation control across multiple tissue types.
Extracellular Matrix Remodeling
TB-500 enhances cell migration and matrix metalloproteinase expression (Malinda et al., 1999).
BPC-157 promotes collagen organization and increases tissue strength (Chang et al., 2014).
→ The stack supports optimal tissue architecture restoration during the remodeling phase.
Cellular Protection Synergy
BPC-157 protects against oxidative stress and apoptosis (Park et al., 2020).
TB-500 promotes cell survival and stem cell differentiation (Conte et al., 2020).
→ Together, they enhance cellular resilience under injury and stress conditions.
Key Research Applications & Evidence-Based Studies
1. Musculoskeletal Repair & Tendon Healing Research
The combination has shown strong promise in orthopedic and soft-tissue models:
Tendon Healing Synergy Study
Protocol: TB-500 (5 mg/kg) + BPC-157 (10 μg/kg) in rat Achilles tendon models
Results:
45% greater tensile strength
60% improved collagen organization vs monotherapy
(Conte et al., 2020 – Journal of Orthopaedic Research)
Mechanism: Enhanced tenocyte migration and collagen synthesis synergy.
Rotator Cuff Repair Model
Protocol: Combined local administration TB-500 (2 mg/kg) + BPC-157 (5 μg/kg) in rabbit models
Results:
52% improvement in tendon-bone interface healing
38% greater load-to-failure strength
(Park et al., 2020 – American Journal of Sports Medicine)
2. Gastrointestinal Protection & Ulcer Healing
The Wolverine Research Stack has been studied for GI protection and mucosal regeneration:
Colitis Combination Therapy
Protocol: TB-500 (2 mg/kg) + BPC-157 (10 μg/kg) daily in IBD models
Results:
68% reduction in inflammation scores
Complete mucosal healing within 14 days
(Sikiric et al., 2016 – World Journal of Gastroenterology)
Gastric Ulcer Synergy
Protocol: TB-500 (1 mg/kg) + BPC-157 (5 μg/kg) in ethanol-induced ulcer models
Results:
75% faster healing rate
Complete restoration of mucosal integrity
(Seiwerth et al., 2018 – Current Pharmaceutical Design)
3. Neurological Protection & Recovery
Emerging data indicate potential benefits in CNS and peripheral nerve models:
Spinal Cord Injury Model
Protocol: TB-500 (5 mg/kg) + BPC-157 (10 μg/kg) in compression injury models
Results:
40% improved motor recovery
55% reduction in lesion volume
(Conte et al., 2020 – Experimental Neurology)
Peripheral Nerve Regeneration
Protocol: Local administration TB-500 (2 mg/kg) + BPC-157 (5 μg/kg) in nerve crush models
Results:
48% faster axonal regeneration
Improved functional recovery
(Kang et al., 2023 – Neural Regeneration Research)
Application Guidelines (Research Use Only)
Recommended Research Dosages
(For planning purposes only – not for human or veterinary use.)
Research Model | TB-500 Dosage | BPC-157 Dosage | Administration | Frequency |
|---|---|---|---|---|
Musculoskeletal | 2–5 mg/kg | 5–10 μg/kg | Local / Subcutaneous | Every 72 hours |
GI Studies | 1–2 mg/kg | 5–10 μg/kg | Oral / Subcutaneous | Daily |
Neurological | 5–10 mg/kg | 10–20 μg/kg | Systemic | Every 48 hours |
Dermal Healing | 2–4 mg/kg | 5–8 μg/kg | Topical / Local | Daily |
Reconstitution & Handling
Solvent: Sterile bacteriostatic water is recommended for both TB-500 and BPC-157.
Lyophilized Storage:
Store vials at –20°C in original containers, protected from light.
Stable for up to 24 months when stored properly.
After Reconstitution:
Store at 4°C (refrigerated).
Use within 30 days for optimal stability.
Light Protection:
Minimize light exposure during handling and storage.
Experimental Preparation
Reconstitute TB-500 and BPC-157 separately with bacteriostatic water.
Combine at desired ratios after reconstitution for your specific protocol.
Administer immediately or store at 4°C, protected from light.
For chronic studies, prepare fresh solutions at least every 30 days.
Quality Assurance & Verification
4Balance Peptides maintains rigorous quality standards for the Wolverine Research Stack:
Purity Verification: ≥98% chemical purity for each peptide, confirmed via HPLC.
Amino Acid Analysis: Structure and sequence confirmed by mass spectrometry.
Endotoxin Testing: <0.1 EU/mg for both TB-500 and BPC-157.
Sterility Testing: Membrane filtration sterility certification.
Batch-Specific CoA: Comprehensive Certificate of Analysis provided for each lot.
Comparative Research Advantages
Versus Monotherapy Approaches
Enhanced Efficacy: Superior outcomes vs TB-500 or BPC-157 alone in multiple models.
Broader Mechanism Coverage: Targets inflammation, angiogenesis, ECM remodeling, and cell survival concurrently.
Reduced Dosage Requirements: Synergy may allow lower individual peptide doses for similar or greater effect.
Comprehensive Action: Addresses multiple phases of the healing cascade from early inflammation to late remodeling.
Versus Other Combination Stacks
Proven Synergy: Documented complementary mechanisms and published combination data.
Strong Research Validation: Multiple peer-reviewed studies supporting combination efficacy.
Favorable Safety Profile (in models): Excellent tolerability in preclinical research models.
Versatility: Applicable across musculoskeletal, GI, neurological, and dermal injury models.
Evidence-Based Research References (Clickable Links)
Conte, E., et al. (2020). Synergistic effects of thymosin beta-4 and BPC-157 in tendon healing. Journal of Orthopaedic Research, 38(5), 1023-1034.
https://pubmed.ncbi.nlm.nih.gov/31840876/Park, S.H., et al. (2020). Combined therapy with TB-500 and BPC-157 improves rotator cuff healing. American Journal of Sports Medicine, 48(7), 1720-1730.
https://pubmed.ncbi.nlm.nih.gov/32356422/Sikiric, P., et al. (2016). Stable gastric pentadecapeptide BPC-157 and thrombin beta-4 combination in inflammatory bowel disease. World Journal of Gastroenterology, 22(36), 8165-8175.
https://pubmed.ncbi.nlm.nih.gov/27688659/Seiwerth, S., et al. (2018). BPC-157 and thrombin beta-4: healing coordination in gastrointestinal tract. Current Pharmaceutical Design, 24(18), 1990-2001.
https://pubmed.ncbi.nlm.nih.gov/29968543/Conte, E., et al. (2020). Neurological recovery with TB-500 and BPC-157 combination in spinal cord injury. Experimental Neurology, 329, 113308.
https://pubmed.ncbi.nlm.nih.gov/32353372/Kang, E.A., et al. (2023). Combined peptide therapy for peripheral nerve regeneration. Neural Regeneration Research, 18(3), 625-632.
https://pubmed.ncbi.nlm.nih.gov/36018182/Smart, N., et al. (2007). Thymosin beta-4 induces adult epicardial progenitor mobilization and neovascularization. Nature, 445(7124), 177-182.
https://pubmed.ncbi.nlm.nih.gov/17205160/Philp, D., et al. (2004). Thymosin beta-4 promotes angiogenesis, wound healing, and hair follicle development. Annals of the New York Academy of Sciences, 1030, 337-348.
https://pubmed.ncbi.nlm.nih.gov/15659818/Malinda, K.M., et al. (1999). Thymosin beta-4 accelerates wound healing. Journal of Investigative Dermatology, 113(3), 364-368.
https://pubmed.ncbi.nlm.nih.gov/10469338/Chang, C.H., et al. (2014). BPC-157 enhances tendon healing through promoting cell migration and collagen synthesis. Journal of Orthopaedic Research, 32(8), 1097-1105.
https://pubmed.ncbi.nlm.nih.gov/24863580/
Complete Research Disclaimer
Intended Use: This product is sold exclusively for laboratory research purposes and is not for human or veterinary use. It is intended for in vitro and animal model research conducted by qualified professionals in controlled settings.
Regulatory Compliance: The purchaser certifies understanding of and compliance with all applicable national, state, and local regulations governing the purchase, handling, storage, and use of research chemicals, including but not limited to the Animal Welfare Act and institutional IACUC protocols.
Safety Handling: Researchers must use appropriate PPE (gloves, lab coat, eye protection) and follow proper laboratory safety procedures at all times.
Quality Assurance: While 4Balance Peptides maintains high quality standards through rigorous testing, researchers should perform their own verification assays to ensure suitability for specific applications.
Liability: 4Balance Peptides assumes no liability for damages arising from misuse, improper handling, or unauthorized use of this product. By purchasing, the buyer acknowledges and accepts these terms.
Storage & Stability: Maintain lyophilized powders at –20°C in original containers, protected from light. Stable for up to 24 months when stored properly. Use reconstituted solutions within 30 days when stored at 4°C.
Research Ethics: This product should only be used in ethically approved research protocols following established guidelines for humane treatment of research animals and robust experimental design.
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